รายละเอียดวารสารวิชาการ

ชื่องานวิจัย ( Article Name)

Screening and optimization of mycodextanase for various rare sugar productions.

บทคัดย่อ ( Abstract )

Thirty-nine isolation including 5 bacteria and 34 actinomycetes from soil samples and stock cultures at Chiang mai, Thailand were screened for mycodextranase production using nigeran agar assay incubated at 28 C, 37 C and 45 C for 2 to 12 days. Optimal condition for the enzyme production was 37 C and 45 C for 7 days, the bacteria isolate CMUP1 showd distinctive clear zone-producing isolation. Enzyme activity at 28 C, 37 C and 45 C of this isolate using LB medium revealed equally with Bacillus circulan (positive mycodextranase producer) of 0.062 U/ml. However, CMUP1 was an isolate that giving higest activity of the enzyme in most media and incubation conditions. The isolate was prrliminarily identified as Bacillus sp. Nigerose was a mainly component after hydrolysis of mycodextran by isolated mycodextranase, which was gave glucose is a main component of nigarose degradatin by alpa (1-3) glucosidase, and and glucose can be starting material to producr rare sugar such as L-fructose, L-psicose and allitol(D,L-allitol) via Izumoring parthway. The enzyme activity of some selected isolates compared with the positive strain varied depending om the media components based on Packett-Burman experimental design.

เอกสารวิจัย ( Paper )

VIEW

ผู้เขียน ( Authors )

  • พิชามญชุ์ น้อยสุวรรณ
  • Goro Takata
  • Keiko Uechi
  • Chartchai Khanongnuch
  • Wasu Pathom-aree
  • Saisamorn Lumyong

\