ชื่องานวิจัย ( Article Name )
5
Skin damage in animal model using the UVB-box
บทคัดย่อ ( Abstract )
Nowadays, an in vivo animal model has been widely used to investigate the biological activities of an interested substance. In this study, a mouse model was utilized for the assessment on the protective effect of the interested natural product(s) on UVB-induced skin damage. Hence, UVB-box model was developed to modify the experimental procedures to prevent blindness and minimize the distress of mice during UVB radiation. Our model has been under approval from Center for Animal Research (no.600707), Naresuan University. The designed number of hair-shaved mice on the dorsal back was anesthetized and laid down in the UVB box. The mice’s head was jutted around the box. The inside UVB-box area was W×L×H; 20×72×22(cm). It consisted of 4 UVB lamps (alpha type, 18W) which has wavelength 280- 320 nm. The UVB-box was modified with wooden material that can prevent from UVB spreading. Measurement of UVB intensity was performed by UV meter to calculate the exposure time. After UVB radiation, dorsal mice skin tissues were collected and analyzed by histological techniques. And their structural morphology changes were observed under microscope to determine the hyperplasia as compare to the non-UVB irradiated mice (control group). This model can compromise the UVB exposure directly to avoid the blindness of the mice. Also, they were exposed to constantly stabilized UVB irradiation.
เเอกสารบทความวิจัย ( Paper )
ผู้เขียน ( Authors )
- Suchada Chumsang
- Preeyawass Phimnuan
- Wongnapa Nakyai
- Jarupa Viyoch
ข้อมูลวารสาร ( Proceeding Infomation )
ข้อมูลการประชุม
วันที่อัพเดตข้อมูลล่าสุด
2023-06-21 09:29:07