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ชื่องานวิจัย ( Article Name)

Optimization of glycinebetaine production under salt stress by unicellular cyanobacterium Synechococcus sp. MH 393765

บทคัดย่อ ( Abstract )

This research aims to study the level of NaCl and choline on glycinebetain production and the activity of Betaine aldehyde dehydrogenase and choline dehydrogenase on salt stress condition of Synechococcus sp. It was found that the activity of choline dehydrogenase and activity of Betaine aldehyde dehydrogenase of Synechococcus sp. was enhanced when the choline was added in medium and cultured growth for 9 days. However, the concentration of choline at 50 mM did not increase the glycinebetain. The optimum concentration of choline was 20 mM of choline using the addition of NaCl medium for 0.5 M to accumulate amount of glycinebetaine of 1,986.786 + 187.839 ug/mL. The highest activity of choline dehydrogenase was 4.65 + 0.07 U/mg protein while the highest activity of betaine aldehyde dehydrogenase was 4.99 + 0.02 U/mg protein. The results of this study indicated that high level of NaCl and choline was not affect to increase the accumulation of glycinebetaine.

เอกสารวิจัย ( Paper )

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ผู้เขียน ( Authors )

  • เพ็ชร สุธิภรณ์
  • ฤทัยรัตน์ สุทธิสุวรรณ